Lab Automation
Automated mobile phase and buffer preparation system
Automated mobile phase and buffer preparation for HPLC and tablet dissolution media.
The Acculize MobiPhase One is a lab automation system for mobile phase and buffer preparation in regulated QC and R&D chromatography laboratories. Buffer preparation, pH control, filtration and degassing run in one protocol-driven cycle.
It prepares mobile phases 3x to 5x faster than manual, depending on the method and complexity, and the analyst is free while the run completes.
The same buffer preparation system can also prepare buffers for tablet dissolution media, up to 10 L.
Key Features
- Mobile phases 3x to 5x faster than manual, depending on the method and complexity
- Also prepares buffers for tablet dissolution media, up to 10 L
- Quick method transfer between R&D and Production
- Analyst loads and retrieves; the run completes unattended
- 0.1% error and RSD under 0.1% at 1000 mL
- pH control within ±0.02 pH
- Filtered and degassed, ready to use on completion
- Designed for use in 21 CFR Part 11-regulated environments
One system, two applications
Mobile phase preparation for chromatography
Automated mobile phase prep for HPLC, UPLC and LC-MS: buffer preparation, solvent mixing, pH correction, filtration and degassing, ready to use on completion.
Buffer preparation for tablet dissolution media
Use the same buffer preparation system to prepare buffers for tablet dissolution media, up to 10 L, so one instrument can serve both your chromatography and dissolution work.
Manual mobile phase preparation is slow, variable and hard to audit
Slow
About 90 min to 135 min per mobile phase, before every sequence.
Error-prone
7 to 8 manual steps: weigh, dissolve, adjust pH, filter and degas.
Analyst-dependent
Manual RSD of about 1 to 2%, with variability across analysts and sites.
Wasteful
Excess volumes are prepared to avoid repeating the process, adding solvent and disposal burden.
Hard to audit
Paper logbooks and manual records create data integrity and audit exposure.
Unsafe
Operators are exposed to hazardous solvents.
Industry studies attribute up to 80% of process deviations to human error, with each deviation costing EUR 22,000 to 48,000. Source: PwC
Where the savings come from
| Cost driver | Manual | Acculize MobiPhase One |
|---|---|---|
| Analyst time | Occupied for the full preparation | Load and retrieve; free while the run completes |
| Solvents and buffers | Excess batches | Exact volume, on demand |
| Re-preparation and investigations | Recurring | Reduced by locked, repeatable protocols |
| Consumables | Membrane filters | Membrane filters |
| Maintenance | Not applicable | Locally serviceable components |
On-demand, protocol-driven volumes mean exact preparation, minimal re-preparation caused by pH or ratio errors, lower disposal cost and lower operator exposure.
Four variants, one platform
5 L, single mixing
R&D, method development
5 L, dual mixing
R&D, higher throughput
10 L, single mixing
Production QC
10 L, dual mixing
Production QC, higher throughput
Dual mixing prepares Mobile Phase A and B in parallel, suited to gradient methods. All variants share the same software, protocols and validation package for easy methods transfer between R&D and QC teams. The system is configurable for different buffers, solvent systems and protocols without redesign.
See the chromatograms: Manual vs Acculize MobiPhase One
Request our pilot study report. It compares chromatograms of manual and Acculize MobiPhase One preparations for different molecules, with details of the buffer prepared, pH adjustment and solvent ratios.
Read how we approach data integrity on our Quality & Compliance page, or see our Acculize BET 96 Plus dry bath for 21 CFR Part 11-regulated LAL testing.
Technical Specifications
| Applications | Mobile phase and buffer preparation for HPLC, UPLC and LC-MS; buffers for tablet dissolution media, up to 10 L |
|---|---|
| Accuracy | 1000 mL: 0.1% error, RSD under 0.1% |
| pH control | Within ±0.02 pH |
| Workflow | Buffer preparation, solvent mixing, pH correction, filtration and degassing |
| Preparation speed | 3x to 5x faster than manual, depending on the method and complexity |
| Variants | 5 L or 10 L, each with single or dual mixing (A and B in parallel) |
| Software | Designed for use in 21 CFR Part 11-regulated environments: user access levels, e-signatures, audit trails, protocol locking, PDF reports, LIMS compatibility |
| Included | Installation, IQ/OQ documentation, training and warranty |
Frequently Asked Questions
The Acculize MobiPhase One automates mobile phase and buffer preparation for regulated QC and R&D laboratories: buffer preparation, solvent mixing, pH correction, filtration and degassing, with electronic records of every step. The mobile phase is ready to use on completion.
Yes. The buffer preparation system within the Acculize MobiPhase One can prepare buffers for tablet dissolution media, up to 10 L. Labs that run both chromatography and dissolution testing can use one system for both.
The Acculize MobiPhase One prepares mobile phases 3x to 5x faster than manual preparation, depending on the method and complexity. The analyst loads and retrieves, and is free while the run completes.
At 1000 mL, it achieves 0.1% error with RSD under 0.1%, compared with a manual RSD of about 1 to 2%. pH is controlled to within ±0.02 pH.
Four variants on one platform: 5 L or 10 L, each with single or dual mixing. Dual mixing prepares Mobile Phase A and B in parallel, which suits gradient methods. All variants share the same software, protocols and validation package, so methods transfer between R&D and QC.
The Acculize MobiPhase One is designed for use in 21 CFR Part 11-regulated environments, with user access levels, e-signatures, audit trails, protocol locking and PDF reports. The software is GAMP 5 Category 4 (Configured Software). Regulatory compliance depends on the user's validated system and procedures; Acculize lab devices provide the technical controls and documentation to support it.
Yes. Request our pilot study report on this page. It compares chromatograms of manual and Acculize MobiPhase One preparations for different molecules.
Yes. Acculize Innovations is based in Chennai, India, and each unit includes installation, IQ/OQ documentation, training and warranty. Contact us for mobile phase automation and automated buffer prep for your lab, in India or abroad.
Each unit includes installation, IQ/OQ documentation, training and warranty. Book a demo to see a run and discuss your methods and volumes.
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